Journal: PLoS ONE

Article Title: Reduction of the HIV Protease Inhibitor-Induced ER Stress and Inflammatory Response by Raltegravir in Macrophages

doi: 10.1371/journal.pone.0090856

Figure Lengend Snippet: Real time PCR primers.

Article Snippet: Mouse monoclonal antibody against CD36 was from Cayman Chemical (Ann Arbor, MI).

Techniques: Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Reduction of the HIV Protease Inhibitor-Induced ER Stress and Inflammatory Response by Raltegravir in Macrophages

doi: 10.1371/journal.pone.0090856

Figure Lengend Snippet: ( A–C ) Human THP-1-derived macrophages were treated with HIV PIs (lopinavir/Ritonavir, LOPV/RITV, 25 µM) with or without raltegravir (25 µM) for 24 h. The total cellular RNA was isolated and reverse transcribed. The relative mRNA levels of CD36, SRA, and LDLR were determined by real-time PCR as described in “Methods”. The values are means ± S.E. of three independent experiments. **, p<0.01, ***, p<0.001, statistical significance relative to vehicle control. #, p<0.05, statistical significance of HIV PI+raltegravir-treated group relative to HIV PI-treated group. ( D ) Human THP-1-derived macrophages were treated with HIV PIs (lopinavir/Ritonavir, LOPV/RITV, 25 µM) with or without raltegravir (25 µM) for 24 h. The total protein lysates were prepared and subjected to Western blot analysis. Representative immunoblots for CD36, SRA and β-actin are shown. β-actin was used as the loading control for total proteins.

Article Snippet: Mouse monoclonal antibody against CD36 was from Cayman Chemical (Ann Arbor, MI).

Techniques: Derivative Assay, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Western Blot

Journal: Frontiers in Immunology

Article Title: Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing

doi: 10.3389/fimmu.2025.1521554

Figure Lengend Snippet: Screening hub genes related to efferocytosis in diabetic kidney macrophages. The GO enrichment analysis of macrophage subpopulations in 3-month-old (A) and 7-month-old mice (B) . (C) The chord diagram about the interaction among renal immune cells. (D) The Venn diagram illustrating the intersection between GSE195799 and the gene list of efferocytosis. (E) The PPI diagram, where the color varies from light to dark, signifies an ascending order of degree. (F–H) Violin diagrams of the distribution of CD36, ITGAM, and CX3CR1 in each cluster. GO, gene ontology; MIF, macrophage migration inhibitory factor; PPI, protein-protein interaction network.

Article Snippet: Afterwards, the primary antibody incubation was performed at 4°C overnight, encompassing antibodies for CD36 (A19016, ABclonal, China), ITGAM (ab133357, Abcam, Cambridge, UK), and CX3CR1 (13885-1-AP, Proteintech, China).

Techniques: Migration

Journal: Frontiers in Immunology

Article Title: Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing

doi: 10.3389/fimmu.2025.1521554

Figure Lengend Snippet: Validating the clinical relevance of hub molecules. (A–C) illustrating the distribution characteristics of the data via violin plots. Scatter plots describe the correlation between CD36 (D) , ITGAM (E) , and CX3CR1 (F) expression with clinical indicators. ***P<0.001.

Article Snippet: Afterwards, the primary antibody incubation was performed at 4°C overnight, encompassing antibodies for CD36 (A19016, ABclonal, China), ITGAM (ab133357, Abcam, Cambridge, UK), and CX3CR1 (13885-1-AP, Proteintech, China).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Unveiling macrophage dynamics and efferocytosis-related targets in diabetic kidney disease: insights from single-cell and bulk RNA-sequencing

doi: 10.3389/fimmu.2025.1521554

Figure Lengend Snippet: Evaluation of target gene expression in DKD model. (A) Quantitative PCR analysis of ITGAM, CD36, and CX3CR1 mRNA expression. (B) Western blot showing the protein expression levels of ITGAM, CD36, and CX3CR1. (C) Quantitative analysis of protein band grayscale values for ITGAM, CD36, and CX3CR1. *P < 0.05, **P < 0.01, ***P<0.001.

Article Snippet: Afterwards, the primary antibody incubation was performed at 4°C overnight, encompassing antibodies for CD36 (A19016, ABclonal, China), ITGAM (ab133357, Abcam, Cambridge, UK), and CX3CR1 (13885-1-AP, Proteintech, China).

Techniques: Targeted Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Western Blot

Journal: Frontiers in Endocrinology

Article Title: The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes

doi: 10.3389/fendo.2018.00616

Figure Lengend Snippet: Protein expression of FFA protein transporters. The effect of mild (4% O 2 ) and severe (1% O 2 ) hypoxia (A) and metformin administration (B) on CD36 and SCL27A4 protein expression. * p < 0.05 for comparison with control exposures (20% O 2 , unpaired T -test), # p < 0.05 for comparison with vehicle-treated group (unpaired T -test), n = 6–9.

Article Snippet: Western blotting: Protein expression of SLC27A4, CD36, and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase—a loading control) was performed by western blot analysis using rabbit polyclonal antibodies (Anti-SLC27A4 antibody: ab199718, Anti-CD36 antibody: ab133625, and Anti-GAPDH antibody: ab9485, all from Abcam, Cambridge, UK).

Techniques: Expressing

Journal: Frontiers in Endocrinology

Article Title: The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes

doi: 10.3389/fendo.2018.00616

Figure Lengend Snippet: Gene expression of FFA protein transporters and key regulators of mitochondria metabolism. The effect of mild (4% O 2 ) and severe (1% O 2 ) hypoxia (A) and metformin administration (B) on CD36, SCL27A4, CPT1, UCP2, and PGC1α gene expression. * p < 0.05 for comparison with control exposures (20% O 2 ), # p < 0.05 for comparison with vehicle-treated group, n = 6. Gene expression data were analyzed using the REST Software (Qiagen, Hilden, Germany) based on the Pfaffl's method.

Article Snippet: Western blotting: Protein expression of SLC27A4, CD36, and GAPDH (Glyceraldehyde 3-phosphate dehydrogenase—a loading control) was performed by western blot analysis using rabbit polyclonal antibodies (Anti-SLC27A4 antibody: ab199718, Anti-CD36 antibody: ab133625, and Anti-GAPDH antibody: ab9485, all from Abcam, Cambridge, UK).

Techniques: Expressing, Software